產地 | 美國 |
品牌 | GE |
貨號 | 07851 |
用途 | 分離人外周血液 |
包裝規格 | 500ml |
純度 | % |
是否進口 | 否 |
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TEMCELL淋巴細胞分離液Lymphoprep 現貨促銷中!
產品型號: 07851
STEMCELL淋巴細胞分離液Lymphoprep
*替代Ficoll-Paque
工作原理
PBMC與血液中的其他成分存在密度差異,利用密度在1.077±0.001g/ml之間而且近于等滲的LymphoprepTM溶液作密度梯度離心時,紅細胞和粒細胞由于密度大,故沉于分離液底部。PBMC密度稍低于分離液,故位于分離液界面上,獲得PBMC。LymphoprepTM分離液含有多糖(polysacccharide),多糖能粘附在細胞表面,使紅細胞和粒細胞聚集下沉,但它對淋巴細胞的活性和紅細胞和粒細胞進一步分離有負面影響。
STEMCELL淋巴細胞分離液Lymphoprep
注意事項:
1、該分離液僅適用于人體的外周血液,不適用于動物。
2、使用該分離液時,需將待分離的血液用生理鹽水做等體積的稀釋。
3、分離液的保存應置于無菌環境,并避光保存。
4、詳細操作步驟見產品說明書。
技術參數
成分:泛影葡胺鈉(Sodium diatrizoate):9.1% (w/v);多糖:5.7% (w/v)
密度:1.077±0.001g/ml
滲透性:290±15mOsm
內毒素含量:<1.0EU/ml
分離所得PBMC中紅細胞的含量:整個細胞數目的3-10%。
Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Granulocytes and erythrocytes have a higher density than mononuclear cells and therefore sediment through the Lymphoprep™ layer during centrifugation. Lymphoprep can be substituted for Ficoll-Paque™ without any need to change your existing protocols and is fully compatible with both Sepmate™ and RosetteSep™.
Product Type: | Cell isolation products |
Recommended For: | Isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow |
Components: | ? Sodium Diatrizoate 9.1% (w/v) ? Polysaccharide 5.7% (w/v) ? Water ? Density of 1.077 g/mL |
Accessory Products: | SepMate™-50 (Catalog #15450/15460) |
Contains: | ? Sodium Diatrizoate (9.1% w/v) ? Polysaccharide (5.7% w/v) ? Density of 1.077 g/mL |
Equipment Required: | Centrifuge |
Cell Isolation Product Type: | Reagents |
Cell Source: | Bone marrow, Cord blood, Whole blood |
Legal Statement: | Lymphoprep™ is a trademark of Axis-Shield. |
Intended Use Statement: | FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. |
ISO Statement: | STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS. |
訂購信息:
07851 500ml
07861 6*500ml
買試劑,找華雅,更多生物試劑,就在華雅思創??!!
TEMCELL淋巴細胞分離液Lymphoprep 現貨促銷中!
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